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ConA Lectin (Biotin)
品牌: GeneTex
产品货号: GTX01504
规格: 1mg

报价:
折后价:

数量: - +
产品详情
* 以下信息仅供参考,详情请以原厂网站为准
  • 产品名称:
    ConA Lectin (Biotin)
  • 别名:
    Concanavalin A , Concanavalin A , Canavalia ensiformis , Canavalia ensiformis lectin , ConA lectin , Canavalia ensiformis , ConA , Concanavalin A lectin , Con A lectin
  • 产品描述:
    Concanavalin A is a lectin protein (MW 104kDa), homotetramer 26 kDa; originally extracted from the jack-bean, Canavalia ensiformis. It binds specifically to certain structures found in various sugars α-mannosyl and α-glucosyl residues in glycoproteins. It was the first lectin to be available on a commercial basis and is widely used in biology and biochemistry to characterize glycoproteins and other sugar-containing entities. It is also used to purify macromolecules in lectin affinity chromatography. Concanavalin A interacts with diverse receptors containing mannose carbohydrates (serum and membrane glycoproteins).ConA agglutinate strongly erythrocytes without being blood group specific. Normal cell re agglutinated after trypsinisation. ConA is not also a lymphocyte mitogen and reacts with many bacteria, like E. coli Dictyostelium discoideum et B. substilis, but also widely believed to be involved in the interaction between alpha-mannosyl oligosaccharides on the surface of the HIV virus and the human T cell lymphocyte.
  • 反应种属:
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  • 实验应用:
    ELISA, IHC, WB
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  • 偶联:
    Biotin
  • 产品浓度:
    5 mg/ml (Please refer to the vial label for the specific concentration.)
  • 表达系统:
    Native Protein
  • 阳性对照:
  • 保存温度:
    Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
  • 运输温度:
    4°C
  • 细胞定位:
  • 预期分子量:
  • 产品形式:
    Liquid
  • 存储溶液:
    10mM Bicarbonate, 150mM NaCl, 0.1mM Calcium Chloride, 0.01mM Manganese Chloride (pH8.0). (Con A has an Isoelectric point of about pH4.5-5.5, requires calcium or manganese ions at each of its four saccharide binding sites. These ions should be used in buffer. DO NOT USE Phosphate buffer for dilution of this lectin as it will decrease the activity of lectin).
  • 有效期:
  • 产地:
    美国
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  • 功能与背景:
    Concanavalin A is a lectin protein (MW 104kDa), homotetramer 26 kDa; originally extracted from the jack-bean, Canavalia ensiformis. It binds specifically to certain structures found in various sugars α-mannosyl and α-glucosyl residues in glycoproteins. It was the first lectin to be available on a commercial basis and is widely used in biology and biochemistry to characterize glycoproteins and other sugar-containing entities. It is also used to purify macromolecules in lectin affinity chromatography. Concanavalin A interacts with diverse receptors containing mannose carbohydrates (serum and membrane glycoproteins).ConA agglutinate strongly erythrocytes without being blood group specific. Normal cell re agglutinated after trypsinisation. ConA is a also a lymphocyte mitogen.ConA reacts with many bacteria, like E. coli Dictyostelium discoideum et B. substilis It is also widely believed to be involved in the interaction between alpha-mannosyl oligosaccharides on the surface of the HIV virus and the human T cell lymphocyte. Inhibiting/Eluting sugars: 200 α-mM α-methyl mannoside / 200 mM α-methyl glucoside mixture.
    Carbohydrate-Binding Specificity of Con A: (Manα1,2Manα1,2Man > Manα1,2Man > α-Man > α-Glc > αGlcNAc
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  • 样本类型:
    10mM Bicarbonate, 150mM NaCl, 0.1mM Calcium Chloride, 0.01mM Manganese Chloride (pH8.0). (Con A has an Isoelectric point of about pH4.5-5.5, requires calcium or manganese ions at each of its four saccharide binding sites. These ions should be used in buffer. DO NOT USE Phosphate buffer for dilution of this lectin as it will decrease the activity of lectin).
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